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nse  (R&D Systems)


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    Structured Review

    R&D Systems nse
    Nse, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nse/Human%2FMouse%2FRat+Enolase+2%2FNeuron-specific+Enolase+Antibody/pm41639749-82-31-33
    Average 94 stars, based on 6 article reviews
    nse - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Blood and MRI biomarkers of mild Traumatic Brain Injury in non-concussed collegiate football players
    Article Snippet: .. Biomarkers of brain injury including S100 calcium-binding protein B (S100B) (MyBioSource, Inc., USA), brain-derived neurotrophic factor (BDNF) (R&D System, USA), glial brillary acidic protein (GFAP) (R&D System, MA, USA), neuro lament light chain protein (NFL) (MyBioSource, Inc., USA), and neuron speci c enolase (NSE) (R&D System, MA, USA) were measured from serum samples using quantitative Enzyme-Linked Immunosorbent Assays (ELISA). ..

    Article Title: Effects of auriculotherapy on anxiety and biomarkers in Primary Health Care: a clinical trial
    Article Snippet: .. For the assay, 100 μl of sample was used to measure the concentrations of BDNF, S100B, and NSE (DY248, DY1820, DY5169, DuoSet ELISA Human, R&D Systems, Minneapolis, MN, USA), according to the kit manufacturer’s instructions. .. The values obtained were estimated by interpolating the data with a standard curve for each biomarker using a colorimetric assay, measured at 450 nm (wavelength correction 540 nm) in a spectrophotometer (Perlong DNM-9602, Perlong Medical Equipment Co., Nanjing, China), and the results were expressed in picograms per milliliter (pg/mL).

    Article Title: Effects of auriculotherapy on anxiety and biomarkers in Primary Health Care: a clinical trial.
    Article Snippet: .. For the assay, 100 μl of sample was used to measure the concentrations of BDNF, S100B, and NSE (DY248, DY1820, DY5169, DuoSet ELISA Human, R&D Systems, Minneapolis, MN, USA), according to the kit manufacturer’s instructions. .. The values obtained were estimated by interpolating the data with a standard curve for each biomarker using a colorimetric assay, measured at 450 nm (wavelength correction 540 nm) in a spectrophotometer (Perlong DNM-9602, Perlong Medical Equipment Co., Nanjing, China), and the results were expressed in picograms per milliliter (pg/mL).

    Article Title: Stress biomarker changes following a series of repeated static and dynamic apneas in non-divers.
    Article Snippet: hscTnT was quantified by electro-chemiluminescence immunoassay (Cobas E 411 Analyzer; Roche Diagnostics, Mannheim, Germany; intraassay variability ~ 4.3%). .. Enzyme-linked immunosorbent assay (ELISA) was performed to assess serum concentrations of NSE (R&D systems, A. Elia et al. Respiratory Physiology & Neurobiology 323 (2024) 104228 Quantikine IVD ELISA, Human Enolase 2/Neuron-Specific Enolase immunoassay, DENL20; intra-assay variability ~ 3.1%), IMA (BIOMATIK, Ischemia Modified Albumin ELISA, EKU08701; intra-assay variability ~ 6%), and myoglobin (Abcam, Human Myoglobin ELISA, ab171580; intra-assay variability ~ 4.2%). .. All data were statistically analysed using IBM SPSS Statistics software version 28 (IBM Corp., Armonk, NY, USA).

    Article Title: Blood-Brain Barrier Biomarkers before and after Kidney Transplantation.
    Article Snippet: .. Commercially available enzyme-linked immunosorbent assay (ELISA) kits were used to quantify the circulating levels of the biomarkers of NSE (DENL20, R&D systems, Abingdon, UK), BDNF (DBD00, R&D systems, Abingdon, UK), and NfL (EKX-RPTZML-96, Nordic Biosite AB, Täby, Sweden) in the plasma samples, and used according to the manufacturer’s instructions. ..

    Incubation:

    Article Title: Astrocytic CCL5 orchestrates CCR5-positive neuronal necroptosis in subarachnoid hemorrhage
    Article Snippet: 30 μm brain sections encompassing each hippocampus were cut using a cryostat microtome (Leica, CM1900), blocked with immunohistochemistry blocking buffer (Beyotime, p0260), and then incubated with primary antibodies at 4 °C overnight as previously report [ ]. .. The primary antibodies included: CCR5 (ab65850, abcam), GFAP (NB300-141, NOVUS), GFAP (C9205, sigma), NeuN (ab104224, abcam), CX3CR1 (AF5825, R&D Systems), DCX (ab207175, abcam), CCL5 (ab322195,abcam), Iba-1 (ab318302, abcam), p-MLKL (#37333, CST), NSE (AF5169, R&D Systems) overnight at 4 °C and then incubated with relative secondary antibodies for 2 h at room temperature. .. DAPI (4′,6-diamidino-2- phenylindole) ( P36931 , Life Technologies) visualizes nuclei.

    Article Title: Astrocytic CCL5 orchestrates CCR5-positive neuronal necroptosis in subarachnoid hemorrhage.
    Article Snippet: 30 μm brain sections encompassing each hippocampus were cut using a cryostat microtome (Leica, CM1900), blocked with immunohistochemistry blocking buffer (Beyotime, p0260), and then incubated with primary antibodies at 4 °C overnight as previously report [23]. .. The primary antibodies included: CCR5 (ab65850, abcam), GFAP (NB300-141, NOVUS), GFAP (C9205, sigma), NeuN (ab104224, abcam), CX3CR1 (AF5825, R&D Systems), DCX (ab207175, abcam), CCL5 (ab322195,abcam), Iba-1 (ab318302, abcam), p-MLKL (#37333, CST), NSE (AF5169, R&D Systems) overnight at 4 °C and then incubated with relative secondary antibodies for 2 h at room temperature. .. DAPI (4′,6-diamidino-2- phenylindole) (P36931, Life Technologies) visualizes nuclei.

    Intra Assay:

    Article Title: Stress biomarker changes following a series of repeated static and dynamic apneas in non-divers.
    Article Snippet: hscTnT was quantified by electro-chemiluminescence immunoassay (Cobas E 411 Analyzer; Roche Diagnostics, Mannheim, Germany; intraassay variability ~ 4.3%). .. Enzyme-linked immunosorbent assay (ELISA) was performed to assess serum concentrations of NSE (R&D systems, A. Elia et al. Respiratory Physiology & Neurobiology 323 (2024) 104228 Quantikine IVD ELISA, Human Enolase 2/Neuron-Specific Enolase immunoassay, DENL20; intra-assay variability ~ 3.1%), IMA (BIOMATIK, Ischemia Modified Albumin ELISA, EKU08701; intra-assay variability ~ 6%), and myoglobin (Abcam, Human Myoglobin ELISA, ab171580; intra-assay variability ~ 4.2%). .. All data were statistically analysed using IBM SPSS Statistics software version 28 (IBM Corp., Armonk, NY, USA).

    Modification:

    Article Title: Stress biomarker changes following a series of repeated static and dynamic apneas in non-divers.
    Article Snippet: hscTnT was quantified by electro-chemiluminescence immunoassay (Cobas E 411 Analyzer; Roche Diagnostics, Mannheim, Germany; intraassay variability ~ 4.3%). .. Enzyme-linked immunosorbent assay (ELISA) was performed to assess serum concentrations of NSE (R&D systems, A. Elia et al. Respiratory Physiology & Neurobiology 323 (2024) 104228 Quantikine IVD ELISA, Human Enolase 2/Neuron-Specific Enolase immunoassay, DENL20; intra-assay variability ~ 3.1%), IMA (BIOMATIK, Ischemia Modified Albumin ELISA, EKU08701; intra-assay variability ~ 6%), and myoglobin (Abcam, Human Myoglobin ELISA, ab171580; intra-assay variability ~ 4.2%). .. All data were statistically analysed using IBM SPSS Statistics software version 28 (IBM Corp., Armonk, NY, USA).

    Clinical Proteomics:

    Article Title: Blood-Brain Barrier Biomarkers before and after Kidney Transplantation.
    Article Snippet: .. Commercially available enzyme-linked immunosorbent assay (ELISA) kits were used to quantify the circulating levels of the biomarkers of NSE (DENL20, R&D systems, Abingdon, UK), BDNF (DBD00, R&D systems, Abingdon, UK), and NfL (EKX-RPTZML-96, Nordic Biosite AB, Täby, Sweden) in the plasma samples, and used according to the manufacturer’s instructions. ..



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    Image Search Results


    ( A-C ) Quantification of neuron-specific enolase (NSE) levels in brain tissue lysate (A), blood plasma (B), and cerebrospinal fluid (CSF) (C) from wild-type (WT), Nrf2⁻/⁻, and ICAM-1⁻/⁻ mice under control and injury conditions (n = 8/group).( D-F ) Quantification of S100β levels in brain tissue lysate (D), blood plasma (E), and CSF (F) across the same experimental groups (n = 8/group). All values are expressed as mean ± SEM. Statistical analysis was performed using one-way ANOVA in A or two-way ANOVA in B and C followed by Dunnett’s post hoc test. Statistical significance between groups is indicated on the graphs. p < 0.05 statistically significant.

    Journal: bioRxiv

    Article Title: Nrf2 regulates ICAM-1–mediated neutrophil extracellular trap formation after traumatic brain injury

    doi: 10.64898/2026.05.01.722360

    Figure Lengend Snippet: ( A-C ) Quantification of neuron-specific enolase (NSE) levels in brain tissue lysate (A), blood plasma (B), and cerebrospinal fluid (CSF) (C) from wild-type (WT), Nrf2⁻/⁻, and ICAM-1⁻/⁻ mice under control and injury conditions (n = 8/group).( D-F ) Quantification of S100β levels in brain tissue lysate (D), blood plasma (E), and CSF (F) across the same experimental groups (n = 8/group). All values are expressed as mean ± SEM. Statistical analysis was performed using one-way ANOVA in A or two-way ANOVA in B and C followed by Dunnett’s post hoc test. Statistical significance between groups is indicated on the graphs. p < 0.05 statistically significant.

    Article Snippet: Using commercial ELISA kits, the level of S100β (Abnova, Littleton CO, USA) and neuron specific enolase (NSE) (Alpha Diagnostic, San Antonio, Texas, USA; Cat. No 0050) were analyzed in tissue lysates as per manufacturer’s instructions.

    Techniques: Clinical Proteomics, Control